Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Screening lead compounds for antibacterial activity against Gram-positive and Gram-negative bacteria. Two-fold dilutions of six lead compounds (labelled 1–6) were applied in 6 μL volumes to the surface of a soft agar overlay inoculated with Bacillus subtilis , Staphylococcus epidermidis , Escherichia coli or Pseudomonas fluorescens . The LB agar plates were exposed to light at 365 nm for 5 min and then incubated for 24 h at 37°C prior to imaging. Controls without light activation or with application of DMSO are shown in .

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques: Activity Assay, Bacteria, Incubation, Imaging, Activation Assay

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Effect of Compound 2 on bacterial growth. Escherichia coli (A) , B. subtilis (B) and S. epidermidis (C) were cultivated in LB broth at 37°C in 96-well plates in a plate reader with continuous shaking. Growth was monitored at OD 600nm in samples exposed to light at 365 nm for 5 min (filled symbols) or without light treatment (open symbols). Samples contained 2 μM Compound 2 or 0.2% DMSO indicated by circles or squares, respectively.

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques:

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Real-time monitoring of bacterial membrane integrity. The BacLight assay of membrane integrity following photoactivation of Compound 2 . S. epidermidis (A) , B. subtilis (B) , P. fluorescens (C) and E. coli (D) in mid-log phase of growth were stained with PI (magenta) and SYTO 9 (yellow) and imaged by confocal microscopy without light activation (−) or 10 min after photoactivation with the 405 nm laser (+). The laser was applied at 30% power for 1 min. The bar represents 3 μm.

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques: Membrane, BacLight Assay, Staining, Confocal Microscopy, Activation Assay

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Role of the E. coli outer membrane in protecting against Compound 2 toxicity. (A) The viability of E. coli O + , O − and Δ rfaC cells was evaluated as in with exposure to 2 μM Compound 2 with or without light activation. (B) PI assay of E. coli strains to monitor loss of membrane integrity. (C) Real-time imaging of E. coli strains stained with PI (magenta) and SYTO 9 (yellow) by confocal microscopy. Images taken prior to light exposure (−) and 10 min after photoactivation (+) as indicated. The bar represents 3 μm.

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques: Membrane, Activation Assay, Imaging, Staining, Confocal Microscopy

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Susceptibility of E. coli strains deficient in oxidative damage and tolerance pathways. The viability of bacteria exposed to 2 μM Compound 2 after photoactivation is shown. Serial dilutions of bacteria were applied to LB agar plates and colonies counted to determine CFU/ml. A representative image of each strain is shown (A) and the CFU/ml (B) represents the mean and standard error of three independent experiments.

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques: Bacteria

Journal: Food Technology and Biotechnology

Article Title: Autochthonous Human Milk Lactobacillus Strains as Potential Probiotic Starter Cultures

doi: 10.17113/ftb.64.01.26.9074

Figure Lengend Snippet: Antimicrobial activity of lactic acid bacteria (LAB) strains against: a) test microorganisms, and b) related LAB strains tested by the turbidimetric method. Different letters above bars indicate statistically significant difference (p<0.05) between the LAB strains and the same test microorganism, i.e. a related LAB strain

Article Snippet: The antimicrobial activity of the selected LAB isolates from human milk was tested against test microorganisms ( Staphylococcus aureus ATCC ® 25925 TM , Listeria monocytogenes ATCC ® 19111 TM , Escherichia coli ATCC ® 25922 TM and Salmonella Typhimurium FP1) and related LAB strains ( Lactobacillus helveticus M92, L. lactis ssp. lactis LMG 9450 and Enterococcus faecium ATCC ® 9430 TM ) cultured in the appropriate medium until their A 620 nm reached 0.5 and 1.0, respectively.

Techniques: Activity Assay, Bacteria

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Structure of pantothenic acid and N-substituted pantothenamides

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Synthetic route to new N-substituted pantothenamides

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Antibacterial activity of N -substituted pantothenamides 9a–9m

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques: Activity Assay