Journal: Journal of Functional Biomaterials

Article Title: FeMn with Phases of a Degradable Ag Alloy for Residue-Free and Adapted Bioresorbability

doi: 10.3390/jfb13040185

Figure Lengend Snippet: Effect of degradation supernatant on the growth of the reference strain Escherichia coli (ATCC ® 25922). Results represent effects caused by the previous degradation in 3 mL Mueller–Hinton broth. Mean value +/− standard deviation from four replicates. The optical density of the untreated control was set as 100%. Repeated measures analysis of variance (ANOVA) summary with Holm–Šídák’s multiple comparisons test in comparison to control; p < 0.001 = * (GraphPad Prism 9.1.0.; GraphPad Software, LLC, San Diego, CL, USA).

Article Snippet: Standard strains of Escherichia coli ( E. coli ; ATCC ® 25922, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) are used since these bacteria are common etiologic agents of clinical infections involved in bone tissue infections related to implants.

Techniques: Standard Deviation, Control, Comparison, Software

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Structure of pantothenic acid and N-substituted pantothenamides

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Synthetic route to new N-substituted pantothenamides

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Antibacterial activity of N -substituted pantothenamides 9a–9m

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques: Activity Assay

Journal:

Article Title: Constitutive Expression of a Chromosomal Class A (BJM Group 2) ?-Lactamase in Xanthomonas campestris

doi: 10.1128/AAC.48.1.209-215.2004

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Antibiotics were added when necessary: ampicillin (50 μg/ml), kanamycin (50 μg/ml), and tetracycline (15 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Characteristic(s) Source or reference E. coli DH5α endA1 hsdR17 (r k − m k + ) supE44 thi-1 recA gyrA relA1 φ80d lacZ ΔM15 Δ( lacZYA - argF ) Bethesda Research Laboratories ATCC 25922 Reference strain for MIC tests American Type Culture Collection E. coli (pFY13-9BSRX) DH5α with plasmid pFY13-9BSRX carrying the entire ampR1 and bla XCC-1 genes, Km r /Ap s This study E. coli (pBX) DH5α with plasmid pBX carrying the bla XCC-1 promoter situated upstream of the luxA and fluxB reporter genes, Tc r /Ap s This study E. coli (pRX) DH5α with plasmid pRX carrying the ampR1 promoter situated upstream of the luxA and luxB reporter genes, Tc r /Ap s This study X. campestris pv. campestris Xc2 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection a Xc6 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc85 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc17 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11A Spontaneous avirulent mutant from Xc11, Ap r Yang and Tseng ( 33 ) Xc11bla::Tc Xc11 bla XCC-1 mutant constructed by inserting a Tc r cartridge into the Sma I site of the bla XCC-1 gene, Tc r /Ap s Weng et al. ( 29 ) Xc11RM Xc11 ampR1 mutant constructed by inserting a Tc r cartridge into the Sal I site of the ampR1 gene, Tc r /Ap s This study Xc11bla::Tc(pRKSE) Xc11 bla XCC-1 mutant complemented with cloned bla XCC-1 gene, Tc r /Ap r /Km r Weng et al. ( 29 ) Xc11RM(pFJ-ampR1) Xc11 ampR1 mutant complemented with cloned ampR1 gene, Tc r /Ap r /Km r This study X. campestris pv. begoniae 59 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. dieffenbachiae 65 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. mangiferaeindcae 38 Wild-type strain isolated in Taiwan, Ap s Tseng lab collection X. campestris pv. phaseoli 73 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. citri 60 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. glycines 69 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. oryzae pv. oryzae 21 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. vesicatoria Xv64 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xv3240 European strain, Ap s Ojanen et al. ( 20 ) Serratia marcescens 6 Wild-type strain isolated in Taiwan, Ap r L. T. Wu, China Medical University Open in a separate window a Yi-Hsiung Tseng's laboratory collection.

Techniques: Plasmid Preparation, Isolation, Mutagenesis, Construct, Clone Assay

Journal:

Article Title: Constitutive Expression of a Chromosomal Class A (BJM Group 2) ?-Lactamase in Xanthomonas campestris

doi: 10.1128/AAC.48.1.209-215.2004

Figure Lengend Snippet: Inserts of the plasmids carrying blaXCC-1 and/or ampR1 from X. campestris pv. campestris strain 11. The 4.0-kb Sau3A1 fragment in pCH1, the 2.5-kb StuI-XbaI fragment in pFY13-9BSRX, the 1.7-kb EcoRI-SstI fragment in pBKE, the 0.5-kb PstI-SstI fragment in pBKX, the 1.0-kb SmaI-SstI fragment in pOKSS, the 1.3-kb PstI-XbaI fragment in pFJ-ampR1, the 0.5-kb PstI-PvuII fragment in pBX, and the same fragment in the opposite orientation in pRX are shown. The underlined restriction sites are from the multiple cloning sites of the vectors.

Article Snippet: Antibiotics were added when necessary: ampicillin (50 μg/ml), kanamycin (50 μg/ml), and tetracycline (15 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Characteristic(s) Source or reference E. coli DH5α endA1 hsdR17 (r k − m k + ) supE44 thi-1 recA gyrA relA1 φ80d lacZ ΔM15 Δ( lacZYA - argF ) Bethesda Research Laboratories ATCC 25922 Reference strain for MIC tests American Type Culture Collection E. coli (pFY13-9BSRX) DH5α with plasmid pFY13-9BSRX carrying the entire ampR1 and bla XCC-1 genes, Km r /Ap s This study E. coli (pBX) DH5α with plasmid pBX carrying the bla XCC-1 promoter situated upstream of the luxA and fluxB reporter genes, Tc r /Ap s This study E. coli (pRX) DH5α with plasmid pRX carrying the ampR1 promoter situated upstream of the luxA and luxB reporter genes, Tc r /Ap s This study X. campestris pv. campestris Xc2 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection a Xc6 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc85 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xc17 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11 Wild-type strain isolated in Taiwan, Ap r Yang and Tseng ( 33 ) Xc11A Spontaneous avirulent mutant from Xc11, Ap r Yang and Tseng ( 33 ) Xc11bla::Tc Xc11 bla XCC-1 mutant constructed by inserting a Tc r cartridge into the Sma I site of the bla XCC-1 gene, Tc r /Ap s Weng et al. ( 29 ) Xc11RM Xc11 ampR1 mutant constructed by inserting a Tc r cartridge into the Sal I site of the ampR1 gene, Tc r /Ap s This study Xc11bla::Tc(pRKSE) Xc11 bla XCC-1 mutant complemented with cloned bla XCC-1 gene, Tc r /Ap r /Km r Weng et al. ( 29 ) Xc11RM(pFJ-ampR1) Xc11 ampR1 mutant complemented with cloned ampR1 gene, Tc r /Ap r /Km r This study X. campestris pv. begoniae 59 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. dieffenbachiae 65 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. campestris pv. mangiferaeindcae 38 Wild-type strain isolated in Taiwan, Ap s Tseng lab collection X. campestris pv. phaseoli 73 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. citri 60 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. glycines 69 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. oryzae pv. oryzae 21 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection X. axonopodis pv. vesicatoria Xv64 Wild-type strain isolated in Taiwan, Ap r Tseng lab collection Xv3240 European strain, Ap s Ojanen et al. ( 20 ) Serratia marcescens 6 Wild-type strain isolated in Taiwan, Ap r L. T. Wu, China Medical University Open in a separate window a Yi-Hsiung Tseng's laboratory collection.

Techniques: Clone Assay